Real-time PCR quantification of precursor and mature microRNA
نویسندگان
چکیده
منابع مشابه
Real-time PCR quantification of precursor and mature microRNA.
microRNAs (miRNAs) are challenging molecules to amplify by PCR because the miRNA precursor consists of a stable hairpin and the mature miRNA is roughly the size of a standard PCR primer. Despite these difficulties, successful real-time RT-PCR technologies have been developed to amplify and quantify both the precursor and mature microRNA. An overview of real-time PCR technologies developed by us...
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ژورنال
عنوان ژورنال: Methods
سال: 2008
ISSN: 1046-2023
DOI: 10.1016/j.ymeth.2007.09.006